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batchCorrectionPublicData

Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".

Installation of packages

All packages have been compiled in the provided requirements.txt file. Simply use this file to install all pachages via "pip install requirements.txt".

Run example

We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication. Simply execute the main script by running

harmonizedDataCreation.py

Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:

  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods

Data loading and preparation

The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.

The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.

Batch correction

We provide code for the following (optional) batch correction methods:

  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.

Evaluation of the batch correction methods

We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:

  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.

Synthetic data generation

We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.

Contact

This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt. Michael F. AdamerSarah C. Brüningk

About

Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.

Resources

Readme
Activity
Custom properties

Stars

2 stars

Watchers

2 watching

Forks

1 fork
Report repository

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Add copy buttons to all 
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(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '

Skip to content
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages

, 'i');
if (__m === '*' || __re.test(location.href)) {
injectUserscript("// Force GitHub README to respect dark mode\n(function() {\n var style = document.createElement('style');\n style.textContent = '\n .markdown-body {\n color-scheme: dark light;\n }\n .markdown-body pre { background: #161b22 !important; }\n .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; }\n .markdown-body table th, .markdown-body table td { border-color: #30363d !important; }\n .markdown-body img { background: #0d1117; }\n .markdown-body blockquote { border-left-color: #8b949e; }\n .markdown-body hr { border-color: #30363d; }\n ';\n document.head.appendChild(style);\n})();", "GitHub Dark Mode README Fix");
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})();
(function(){
try {
var __m = "*";
var __re = new RegExp('^' + ".*" + '

Skip to content
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages

, 'i');
if (__m === '*' || __re.test(location.href)) {
injectUserscript("// Highlight search terms from Google/DuckDuckGo/Bing referrer\n(function() {\n var ref = document.referrer;\n var terms = [];\n \n if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) {\n var url = new URL(ref);\n var q = url.searchParams.get('q') || url.searchParams.get('p');\n if (q) {\n terms = q.split(/\\s+/).filter(function(t) { return t.length > 2; });\n }\n }\n \n if (terms.length === 0) return;\n \n var style = document.createElement('style');\n style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }';\n document.head.appendChild(style);\n \n function highlight(node) {\n if (node.nodeType === 3) { // text node\n var text = node.textContent;\n var found = false;\n terms.forEach(function(term) {\n var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\\]\\\\]/g, '\\\\') + ')', 'gi');\n if (regex.test(text)) {\n found = true;\n var frag = document.createDocumentFragment();\n var parts = text.split(regex);\n parts.forEach(function(part, i) {\n if (i % 2 === 0) {\n frag.appendChild(document.createTextNode(part));\n } else {\n var span = document.createElement('span');\n span.className = 'userscript-highlight';\n span.textContent = part;\n frag.appendChild(span);\n }\n });\n node.parentNode.replaceChild(frag, node);\n }\n });\n } else if (node.nodeType === 1 && node.childNodes) { // element\n var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT'];\n if (!skipTags.includes(node.tagName)) {\n Array.from(node.childNodes).forEach(highlight);\n }\n }\n }\n \n highlight(document.body);\n \n // Re-highlight on dynamic content\n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1 || node.nodeType === 3) highlight(node);\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Highlight Search Terms");
}
} catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); }
})();
(function(){
try {
var __m = "*";
var __re = new RegExp('^' + ".*" + '

Skip to content
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages

, 'i');
if (__m === '*' || __re.test(location.href)) {
injectUserscript("// Strip utm_, fbclid, gclid, etc. from all links on page\n(function() {\n var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content',\n 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid',\n 'ref', 'ref_src', 'source', 'medium', 'campaign'];\n \n function cleanUrl(url) {\n try {\n var u = new URL(url, window.location.origin);\n var changed = false;\n trackingParams.forEach(function(p) {\n if (u.searchParams.has(p)) {\n u.searchParams.delete(p);\n changed = true;\n }\n });\n return changed ? u.toString() : url;\n } catch (e) {\n return url;\n }\n }\n \n function cleanLinks() {\n document.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n \n cleanLinks();\n \n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1) {\n if (node.tagName === 'A') cleanLinks();\n node.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Remove Tracking Parameters from Links");
}
} catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); }
})();
(function(){
try {
var __m = "youtube.com";
var __re = new RegExp('^' + "youtube\\.com" + '

Skip to content
BranchesTags
Repository files navigation
batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages

, 'i');
if (__m === '*' || __re.test(location.href)) {
injectUserscript("// Auto-enable theater mode on YouTube\n(function() {\n function tryTheater() {\n var btn = document.querySelector('button[aria-label=\"Theater mode\"], ytd-player #player button[title=\"Theater mode\"]');\n if (btn && !btn.classList.contains('activated')) {\n btn.click();\n }\n }\n \n // Try immediately\n tryTheater();\n \n // Try after navigation (SPA)\n var lastUrl = location.href;\n setInterval(function() {\n if (location.href !== lastUrl) {\n lastUrl = location.href;\n setTimeout(tryTheater, 500);\n }\n }, 1000);\n \n // Also try on player load\n var observer = new MutationObserver(tryTheater);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "YouTube Theater Mode Default");
}
} catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); }
})();
(function(){
try {
var __m = "*";
var __re = new RegExp('^' + ".*" + '

Skip to content
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages

, 'i');
if (__m === '*' || __re.test(location.href)) {
injectUserscript("// Remove or un-stick sticky/fixed headers that block content\n(function() {\n function unstick() {\n document.querySelectorAll('header, nav, [role=\"banner\"], .header, .navbar, .sticky, .fixed-top, [style*=\"position: fixed\"], [style*=\"position:sticky\"]').forEach(function(el) {\n if (el.style.position === 'fixed' || el.style.position === 'sticky' || \n getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') {\n el.style.position = 'static';\n el.style.top = 'auto';\n el.style.zIndex = 'auto';\n }\n });\n }\n \n unstick();\n \n var observer = new MutationObserver(unstick);\n observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] });\n})();", "Kill Sticky Headers");
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} catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); }
})();
(function(){
try {
var __m = "*";
var __re = new RegExp('^' + ".*" + '

Skip to content
BranchesTags
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
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batchCorrectionPublicData
Summary of the code published in "reComBat: Batch effect removal in large-scale, multi-source omics data integration".
Installation of packages
All packages have been compiled in the provided requirements.txt file.
Simply use this file to install all pachages via "pip install requirements.txt".
Run example
We provide all data and code to reproduce Figures 1, 2 and S1-S10 of our recent publication.
Simply execute the main script by running
harmonizedDataCreation.py
Here parameter options referring the specific batch correction methods, evaluation metrics and output folders are defined. This script comprises three main parts:
  1. Data loading and metadata preprocessing
  2. Batch correction
  3. Evaluation of the batch correction methods
Data loading and preparation
The relevant data associated with this code is provided as a .zip file and needs to be extracted into the 'data' folder. It comprises >1000 micro array gene expressen samples extracted from the GEO database in October 2020 as indicated by the relevant GSE and GSM identifiers. All data was preprocessed using RMA normalization.
The data annotation (referred to as "metadata") is categorized to reflect the specific PA strain, and culture conditions (temperature, growth medium, culture geometry, antibiotic treatment, growth phase) and each sample is assigned to one of 39 unique metadata subsets (ZeroHops). Only ZeroHops comprising at least 2 batches (GSEs) of at least two samples (GSMs) are kept.
Batch correction
We provide code for the following (optional) batch correction methods:
  1. Uncorrected data
  2. Standardized data (Z-scoreing to mean zero and unit variance was applied)
  3. Marker gene elimination for each of the ZeroHop Clusters (default top 8 marker genes)
  4. Principal component elimination for each of the ZeroHops
  5. reComBat
For each of the relevant methods overview fiures showing t-SNE embeddings of the corrected adata colored by all metadata categories are created to provide a visual inspection of the batch correction success.
Evaluation of the batch correction methods
We provide a range of custom evaluation metrics probing different aspects of a successful batch corrected dataframe. These include:
  1. LDA score
  2. DRS score
  3. Cluster purity and Gini impurity
  4. Minmum Cluster Separation number
  5. Cluster Cross-distance
  6. Logistic Regression (or other classifier) classification performance of batch and ZeroHop.
Synthetic data generation
We also provide code to gerenate and evaluate synthetic data in syntheticDataGeneration.py.Here, the user can define their choice of synthetic data properties, the properties of the imposed batch effects and then correct these with the set of possible methods outlined above. The obtained results can be compared to the relevant ground truth.
Contact
This code is developed and maintained by members of the Machine Learning and Computational Biology Lab of Prof. Dr. Karsten Borgwardt.
Michael F. AdamerSarah C. Brüningk
About
Summary of the code published in 'reComBat: Batch effect removal in large-scale, multi-source omics data integration'.
Resources
Readme
Activity
Custom properties
Stars
2 stars
Watchers
2 watching
Forks
1 fork
Report repository
Releases
Packages
Contributors
Languages