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A two-hour, hands-on introduction to spatial omics for immunologists, run at the
ASI-FIMSA meeting. Everything happens in your browser through Google Colab — there is
nothing to install, and you do not need to be a Python programmer. We look at one
tissue, breast cancer, through three different technologies. You will read
and plot the data yourself, find the tissue niches that make up a tumour
microenvironment, and finish by training a small Vision Transformer that predicts gene
expression straight from an H&E image. Four Tutorials, each self-contained, each a
single notebook you open with one click.
The Tutorials
Click a badge to open that Tutorial in Google Colab. Start with the setup check
before you arrive.
Tutorial
What you do
Rough time
00 · Setup check
Confirms Colab can install the packages and reach the data. (For development and bug fixing, no need to run.)
Not running
01 · Read and visualise
Load and plot data from each of the three platforms (Visium spots, Xenium cells, and the whole-transcriptome Atera) to see what each one can and cannot resolve.
~30 min
02 · Niche analysis
Find tissue niches in the tumour microenvironment (e.g., immune infiltration, tumour boundary, stroma), based on which cells sitting next to which.
~30 min
03 · Cell–cell interaction
Run LIANA+'s ligand–receptor test on proximal cells and watch how a gene panel may limit the questions you can ask from the data.
~30 min
04 · ViT for gene expression
Train a small Vision Transformer that predicts gene expression from H&E morphology alone, then look at which genes it gets right and which it does not.
~30 min
Code and teaching material are MIT licensed — reuse, adapt and teach from
them freely. The data are 10x Genomics datasets under their own terms.