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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

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5 stars

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1 watching

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, 'i'); if (__m === '*' || __re.test(location.href)) { // Add copy buttons to all
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(function() {
function addCopyButtons() {
document.querySelectorAll('pre code').forEach(function(codeBlock) {
if (codeBlock.parentElement.hasAttribute('data-copy-added')) return;
codeBlock.parentElement.setAttribute('data-copy-added', 'true');
var btn = document.createElement('button');
btn.textContent = 'Copy';
btn.style.cssText = 'position:absolute;top:4px;right:4px;padding:2px 8px;font-size:11px;background:#4ecdc4;border:none;border-radius:4px;color:#1a1a2e;cursor:pointer;opacity:0.7;transition:opacity 0.2s;';
btn.onmouseover = function() { this.style.opacity = '1'; };
btn.onmouseout = function() { this.style.opacity = '0.7'; };
btn.onclick = function() {
navigator.clipboard.writeText(codeBlock.textContent).then(function() {
btn.textContent = 'Copied!';
setTimeout(function() { btn.textContent = 'Copy'; }, 1500);
});
};
codeBlock.parentElement.style.position = 'relative';
codeBlock.parentElement.appendChild(btn);
});
}
addCopyButtons();
// Re-run on dynamic content
var observer = new MutationObserver(addCopyButtons);
observer.observe(document.body, { childList: true, subtree: true });
})();
}
} catch(__e) { console.warn('[Userscript:Add Copy Buttons to Code Blocks]', __e); }
})();
(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

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5 stars

Watchers

1 watching

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Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Force GitHub README to respect dark mode (function() { var style = document.createElement('style'); style.textContent = ' .markdown-body { color-scheme: dark light; } .markdown-body pre { background: #161b22 !important; } .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; } .markdown-body table th, .markdown-body table td { border-color: #30363d !important; } .markdown-body img { background: #0d1117; } .markdown-body blockquote { border-left-color: #8b949e; } .markdown-body hr { border-color: #30363d; } '; document.head.appendChild(style); })(); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Highlight search terms from Google/DuckDuckGo/Bing referrer (function() { var ref = document.referrer; var terms = []; if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) { var url = new URL(ref); var q = url.searchParams.get('q') || url.searchParams.get('p'); if (q) { terms = q.split(/\s+/).filter(function(t) { return t.length > 2; }); } } if (terms.length === 0) return; var style = document.createElement('style'); style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }'; document.head.appendChild(style); function highlight(node) { if (node.nodeType === 3) { // text node var text = node.textContent; var found = false; terms.forEach(function(term) { var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\]\\]/g, '\\') + ')', 'gi'); if (regex.test(text)) { found = true; var frag = document.createDocumentFragment(); var parts = text.split(regex); parts.forEach(function(part, i) { if (i % 2 === 0) { frag.appendChild(document.createTextNode(part)); } else { var span = document.createElement('span'); span.className = 'userscript-highlight'; span.textContent = part; frag.appendChild(span); } }); node.parentNode.replaceChild(frag, node); } }); } else if (node.nodeType === 1 && node.childNodes) { // element var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT']; if (!skipTags.includes(node.tagName)) { Array.from(node.childNodes).forEach(highlight); } } } highlight(document.body); // Re-highlight on dynamic content var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1 || node.nodeType === 3) highlight(node); }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Strip utm_, fbclid, gclid, etc. from all links on page (function() { var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content', 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid', 'ref', 'ref_src', 'source', 'medium', 'campaign']; function cleanUrl(url) { try { var u = new URL(url, window.location.origin); var changed = false; trackingParams.forEach(function(p) { if (u.searchParams.has(p)) { u.searchParams.delete(p); changed = true; } }); return changed ? u.toString() : url; } catch (e) { return url; } } function cleanLinks() { document.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } cleanLinks(); var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1) { if (node.tagName === 'A') cleanLinks(); node.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Auto-enable theater mode on YouTube (function() { function tryTheater() { var btn = document.querySelector('button[aria-label="Theater mode"], ytd-player #player button[title="Theater mode"]'); if (btn && !btn.classList.contains('activated')) { btn.click(); } } // Try immediately tryTheater(); // Try after navigation (SPA) var lastUrl = location.href; setInterval(function() { if (location.href !== lastUrl) { lastUrl = location.href; setTimeout(tryTheater, 500); } }, 1000); // Also try on player load var observer = new MutationObserver(tryTheater); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Remove or un-stick sticky/fixed headers that block content (function() { function unstick() { document.querySelectorAll('header, nav, [role="banner"], .header, .navbar, .sticky, .fixed-top, [style*="position: fixed"], [style*="position:sticky"]').forEach(function(el) { if (el.style.position === 'fixed' || el.style.position === 'sticky' || getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') { el.style.position = 'static'; el.style.top = 'auto'; el.style.zIndex = 'auto'; } }); } unstick(); var observer = new MutationObserver(unstick); observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] }); })(); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Universal Dark Mode - works on any site (function() { var enabled = true; function applyDarkMode() { if (!enabled) return; // Create style element if it doesn't exist var style = document.getElementById('universal-dark-mode-style'); if (!style) { style = document.createElement('style'); style.id = 'universal-dark-mode-style'; document.head.appendChild(style); } // Dark mode CSS - inverts colors but preserves images/video style.textContent = ' /* Invert everything except media */ html { filter: invert(1) hue-rotate(180deg) !important; background: #1a1a2e !important; } /* Restore images, videos, iframes, canvas */ img, video, iframe, canvas, svg, picture, [style*="background-image"] { filter: invert(1) hue-rotate(180deg) !important; } /* Preserve specific elements that should not be inverted */ .no-dark-mode, .no-dark-mode *, [data-theme="light"], [data-theme="light"], .ace_editor, .ace_editor *, .CodeMirror, .CodeMirror *, .monaco-editor, .monaco-editor *, .markdown-body pre, .markdown-body pre *, .highlight, .highlight *, pre code, pre code * { filter: none !important; } /* Fix common UI elements */ .modal, .popup, .dropdown-menu, .tooltip, .popover { filter: invert(1) hue-rotate(180deg) !important; background: #2d2d44 !important; border-color: #444 !important; } /* Scrollbars */ ::-webkit-scrollbar { background: #1a1a2e !important; } ::-webkit-scrollbar-thumb { background: #444 !important; } ::-webkit-scrollbar-thumb:hover { background: #555 !important; } /* Selection */ ::selection { background: #4ecdc4 !important; color: #1a1a2e !important; } ::-moz-selection { background: #4ecdc4 !important; color: #1a1a2e !important; } '; } function removeDarkMode() { var style = document.getElementById('universal-dark-mode-style'); if (style) style.remove(); } // Toggle with Alt+Shift+D document.addEventListener('keydown', function(e) { if (e.altKey && e.shiftKey && e.key === 'D') { e.preventDefault(); enabled = !enabled; if (enabled) { applyDarkMode(); console.log('[Universal Dark Mode] Enabled'); } else { removeDarkMode(); console.log('[Universal Dark Mode] Disabled'); } } }); // Apply on load applyDarkMode(); // Re-apply on dynamic content var observer = new MutationObserver(function(mutations) { if (enabled && !document.getElementById('universal-dark-mode-style')) { applyDarkMode(); } }); observer.observe(document.head, { childList: true }); console.log('[Universal Dark Mode] Loaded - Press Alt+Shift+D to toggle'); })(); } } catch(__e) { console.warn('[Userscript:Universal Dark Mode]', __e); } })(); })();
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SpliceR version 1.2.0
Hello! Welcome to SpliceR! We hope that our program can be of use for your design of base editing sgRNAs for protein disruption.
If you are noticing any errors when using the application please feel free to reach out Mitch and Branden at klues009@umn.edu and mori0164@umn.edu for troubleshooting and input on the application.
To run this program online visit this webpage:
z.umn.edu/splicer
To run this program locally please do the following:
1) Download and install R (https://www.r-project.org/)
2) Open the dependencies.R file and run the script. Please note any errors that may occur during running.
3) Copy, paste, and run the following code chunk in your command line:
library(shiny)
library(Biostrings)
library(magrittr)
library(stringi)
library(dplyr)
library(tidyr)
library(ggplot2)
library(grr)
library(printr)
library(plyr)
library(readr)
library(printr)
library(rmarkdown)
library(DT)
library(httr)
library(curl)
3) Determine the directory of the SpliceR app on your computer. It will look something like this:
/Users/Name/Downloads/SpliceR-master
4) Copy, paste, and run the following line of code in your R command line, using the specific directory of the MultiEditR app on your computer:
shiny::runApp(“/Users/Name/Downloads/SpliceR-master”)
5) The application should launch and be used interactively

About

Program for the prediction of BE-splice sgRNAs for base editor mediated knockout

Resources

Stars

5 stars

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages