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tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

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1 star

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1 watching

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var __re = new RegExp('^' + "github\\.com" + '
GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
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tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Force GitHub README to respect dark mode (function() { var style = document.createElement('style'); style.textContent = ' .markdown-body { color-scheme: dark light; } .markdown-body pre { background: #161b22 !important; } .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; } .markdown-body table th, .markdown-body table td { border-color: #30363d !important; } .markdown-body img { background: #0d1117; } .markdown-body blockquote { border-left-color: #8b949e; } .markdown-body hr { border-color: #30363d; } '; document.head.appendChild(style); })(); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + ' GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
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tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Highlight search terms from Google/DuckDuckGo/Bing referrer (function() { var ref = document.referrer; var terms = []; if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) { var url = new URL(ref); var q = url.searchParams.get('q') || url.searchParams.get('p'); if (q) { terms = q.split(/\s+/).filter(function(t) { return t.length > 2; }); } } if (terms.length === 0) return; var style = document.createElement('style'); style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }'; document.head.appendChild(style); function highlight(node) { if (node.nodeType === 3) { // text node var text = node.textContent; var found = false; terms.forEach(function(term) { var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\]\\]/g, '\\') + ')', 'gi'); if (regex.test(text)) { found = true; var frag = document.createDocumentFragment(); var parts = text.split(regex); parts.forEach(function(part, i) { if (i % 2 === 0) { frag.appendChild(document.createTextNode(part)); } else { var span = document.createElement('span'); span.className = 'userscript-highlight'; span.textContent = part; frag.appendChild(span); } }); node.parentNode.replaceChild(frag, node); } }); } else if (node.nodeType === 1 && node.childNodes) { // element var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT']; if (!skipTags.includes(node.tagName)) { Array.from(node.childNodes).forEach(highlight); } } } highlight(document.body); // Re-highlight on dynamic content var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1 || node.nodeType === 3) highlight(node); }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + ' GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
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tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Strip utm_, fbclid, gclid, etc. from all links on page (function() { var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content', 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid', 'ref', 'ref_src', 'source', 'medium', 'campaign']; function cleanUrl(url) { try { var u = new URL(url, window.location.origin); var changed = false; trackingParams.forEach(function(p) { if (u.searchParams.has(p)) { u.searchParams.delete(p); changed = true; } }); return changed ? u.toString() : url; } catch (e) { return url; } } function cleanLinks() { document.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } cleanLinks(); var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1) { if (node.tagName === 'A') cleanLinks(); node.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + ' GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
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tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Auto-enable theater mode on YouTube (function() { function tryTheater() { var btn = document.querySelector('button[aria-label="Theater mode"], ytd-player #player button[title="Theater mode"]'); if (btn && !btn.classList.contains('activated')) { btn.click(); } } // Try immediately tryTheater(); // Try after navigation (SPA) var lastUrl = location.href; setInterval(function() { if (location.href !== lastUrl) { lastUrl = location.href; setTimeout(tryTheater, 500); } }, 1000); // Also try on player load var observer = new MutationObserver(tryTheater); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + ' GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
Skip to content

Repository files navigation

tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { // Remove or un-stick sticky/fixed headers that block content (function() { function unstick() { document.querySelectorAll('header, nav, [role="banner"], .header, .navbar, .sticky, .fixed-top, [style*="position: fixed"], [style*="position:sticky"]').forEach(function(el) { if (el.style.position === 'fixed' || el.style.position === 'sticky' || getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') { el.style.position = 'static'; el.style.top = 'auto'; el.style.zIndex = 'auto'; } }); } unstick(); var observer = new MutationObserver(unstick); observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] }); })(); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); })(); GitHub - krademaker/tagmapAnalyseR: tagmapAnalyseR package for transposon integration mapping (TagMap). · GitHub
Skip to content

Repository files navigation

tagmapAnalyseR

Version 1.0.0, released 20 April 2022.

Stand-alone package integrated into transposon mapping pipeline tagmap_hopping for Tagmentation-Based Mapping (Tagmap) data, as well as stand-alone functionality.

Background

TagmapAnalyseR is a module inside the tagmap_hopping pipeline, and is particularly responsible to map (processed) TagMap reads to genomic locations and figure out where transposons (e.g. PiggyBac or Sleeping Beauty) integrated into the genome. TagmapAnalyseR combines several tricks to derive a consensus mapping location for integrations are read coverage may be ambiguous towards the precise location. More details can be obtained from the author upon request (methods section in thesis).

Usage

Dependencies for pipeline are described in the respective repository, see its map_insertions.R script for practical application of tagmapAnalyseR.

Installation

devtools::install_github("https://github.com/krademaker/tagmapAnalyseR")

Load processed read coverage of (putative) integrations

dt<-tagmapAnalyseR::readPutativeInsertions(input)

Obtain integrations with ambiguous read coverage

ambiguous<-tagmapAnalyseR::findAmbiguousInsertionSites(dt, padding= (nchar(overhang_sequence)*2)+2)

Map integrations

mapped<-tagmapAnalyseR::mapInsertionSites(dt=dt,
bam=bam_path,
overhang=overhang_sequence,
samtoolsPath=samtools_path,
depth= as.integer(minimal_read_depth),
ambiguousInsertions=ambiguous)

Contact / Support

Reach out through the issues section or reach out directly to the author.

About

tagmapAnalyseR package for transposon integration mapping (TagMap).

Resources

Stars

1 star

Watchers

1 watching

Forks

Releases

Packages

Contributors

Languages