Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Add copy buttons to all
 blocks\n(function() {\n function addCopyButtons() {\n document.querySelectorAll('pre code').forEach(function(codeBlock) {\n if (codeBlock.parentElement.hasAttribute('data-copy-added')) return;\n codeBlock.parentElement.setAttribute('data-copy-added', 'true');\n \n var btn = document.createElement('button');\n btn.textContent = 'Copy';\n btn.style.cssText = 'position:absolute;top:4px;right:4px;padding:2px 8px;font-size:11px;background:#4ecdc4;border:none;border-radius:4px;color:#1a1a2e;cursor:pointer;opacity:0.7;transition:opacity 0.2s;';\n btn.onmouseover = function() { this.style.opacity = '1'; };\n btn.onmouseout = function() { this.style.opacity = '0.7'; };\n btn.onclick = function() {\n navigator.clipboard.writeText(codeBlock.textContent).then(function() {\n btn.textContent = 'Copied!';\n setTimeout(function() { btn.textContent = 'Copy'; }, 1500);\n });\n };\n codeBlock.parentElement.style.position = 'relative';\n codeBlock.parentElement.appendChild(btn);\n });\n }\n \n addCopyButtons();\n \n // Re-run on dynamic content\n var observer = new MutationObserver(addCopyButtons);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Add Copy Buttons to Code Blocks");
}
} catch(__e) { console.warn('[Userscript:Add Copy Buttons to Code Blocks]', __e); }
})();
(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Force GitHub README to respect dark mode\n(function() {\n var style = document.createElement('style');\n style.textContent = '\n .markdown-body {\n color-scheme: dark light;\n }\n .markdown-body pre { background: #161b22 !important; }\n .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; }\n .markdown-body table th, .markdown-body table td { border-color: #30363d !important; }\n .markdown-body img { background: #0d1117; }\n .markdown-body blockquote { border-left-color: #8b949e; }\n .markdown-body hr { border-color: #30363d; }\n ';\n document.head.appendChild(style);\n})();", "GitHub Dark Mode README Fix"); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Highlight search terms from Google/DuckDuckGo/Bing referrer\n(function() {\n var ref = document.referrer;\n var terms = [];\n \n if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) {\n var url = new URL(ref);\n var q = url.searchParams.get('q') || url.searchParams.get('p');\n if (q) {\n terms = q.split(/\\s+/).filter(function(t) { return t.length > 2; });\n }\n }\n \n if (terms.length === 0) return;\n \n var style = document.createElement('style');\n style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }';\n document.head.appendChild(style);\n \n function highlight(node) {\n if (node.nodeType === 3) { // text node\n var text = node.textContent;\n var found = false;\n terms.forEach(function(term) {\n var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\\]\\\\]/g, '\\\\') + ')', 'gi');\n if (regex.test(text)) {\n found = true;\n var frag = document.createDocumentFragment();\n var parts = text.split(regex);\n parts.forEach(function(part, i) {\n if (i % 2 === 0) {\n frag.appendChild(document.createTextNode(part));\n } else {\n var span = document.createElement('span');\n span.className = 'userscript-highlight';\n span.textContent = part;\n frag.appendChild(span);\n }\n });\n node.parentNode.replaceChild(frag, node);\n }\n });\n } else if (node.nodeType === 1 && node.childNodes) { // element\n var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT'];\n if (!skipTags.includes(node.tagName)) {\n Array.from(node.childNodes).forEach(highlight);\n }\n }\n }\n \n highlight(document.body);\n \n // Re-highlight on dynamic content\n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1 || node.nodeType === 3) highlight(node);\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Highlight Search Terms"); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Strip utm_, fbclid, gclid, etc. from all links on page\n(function() {\n var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content',\n 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid',\n 'ref', 'ref_src', 'source', 'medium', 'campaign'];\n \n function cleanUrl(url) {\n try {\n var u = new URL(url, window.location.origin);\n var changed = false;\n trackingParams.forEach(function(p) {\n if (u.searchParams.has(p)) {\n u.searchParams.delete(p);\n changed = true;\n }\n });\n return changed ? u.toString() : url;\n } catch (e) {\n return url;\n }\n }\n \n function cleanLinks() {\n document.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n \n cleanLinks();\n \n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1) {\n if (node.tagName === 'A') cleanLinks();\n node.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Remove Tracking Parameters from Links"); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Auto-enable theater mode on YouTube\n(function() {\n function tryTheater() {\n var btn = document.querySelector('button[aria-label=\"Theater mode\"], ytd-player #player button[title=\"Theater mode\"]');\n if (btn && !btn.classList.contains('activated')) {\n btn.click();\n }\n }\n \n // Try immediately\n tryTheater();\n \n // Try after navigation (SPA)\n var lastUrl = location.href;\n setInterval(function() {\n if (location.href !== lastUrl) {\n lastUrl = location.href;\n setTimeout(tryTheater, 500);\n }\n }, 1000);\n \n // Also try on player load\n var observer = new MutationObserver(tryTheater);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "YouTube Theater Mode Default"); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Remove or un-stick sticky/fixed headers that block content\n(function() {\n function unstick() {\n document.querySelectorAll('header, nav, [role=\"banner\"], .header, .navbar, .sticky, .fixed-top, [style*=\"position: fixed\"], [style*=\"position:sticky\"]').forEach(function(el) {\n if (el.style.position === 'fixed' || el.style.position === 'sticky' || \n getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') {\n el.style.position = 'static';\n el.style.top = 'auto';\n el.style.zIndex = 'auto';\n }\n });\n }\n \n unstick();\n \n var observer = new MutationObserver(unstick);\n observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] });\n})();", "Kill Sticky Headers"); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Universal Dark Mode - works on any site\n(function() {\n var enabled = true;\n \n function applyDarkMode() {\n if (!enabled) return;\n \n // Create style element if it doesn't exist\n var style = document.getElementById('universal-dark-mode-style');\n if (!style) {\n style = document.createElement('style');\n style.id = 'universal-dark-mode-style';\n document.head.appendChild(style);\n }\n \n // Dark mode CSS - inverts colors but preserves images/video\n style.textContent = '\n /* Invert everything except media */\n html {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #1a1a2e !important;\n }\n \n /* Restore images, videos, iframes, canvas */\n img, video, iframe, canvas, svg, picture, [style*=\"background-image\"] {\n filter: invert(1) hue-rotate(180deg) !important;\n }\n \n /* Preserve specific elements that should not be inverted */\n .no-dark-mode, .no-dark-mode *,\n [data-theme=\"light\"], [data-theme=\"light\"],\n .ace_editor, .ace_editor *,\n .CodeMirror, .CodeMirror *,\n .monaco-editor, .monaco-editor *,\n .markdown-body pre, .markdown-body pre *,\n .highlight, .highlight *,\n pre code, pre code * {\n filter: none !important;\n }\n \n /* Fix common UI elements */\n .modal, .popup, .dropdown-menu, .tooltip, .popover {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #2d2d44 !important;\n border-color: #444 !important;\n }\n \n /* Scrollbars */\n ::-webkit-scrollbar { background: #1a1a2e !important; }\n ::-webkit-scrollbar-thumb { background: #444 !important; }\n ::-webkit-scrollbar-thumb:hover { background: #555 !important; }\n \n /* Selection */\n ::selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ::-moz-selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ';\n }\n \n function removeDarkMode() {\n var style = document.getElementById('universal-dark-mode-style');\n if (style) style.remove();\n }\n \n // Toggle with Alt+Shift+D\n document.addEventListener('keydown', function(e) {\n if (e.altKey && e.shiftKey && e.key === 'D') {\n e.preventDefault();\n enabled = !enabled;\n if (enabled) {\n applyDarkMode();\n console.log('[Universal Dark Mode] Enabled');\n } else {\n removeDarkMode();\n console.log('[Universal Dark Mode] Disabled');\n }\n }\n });\n \n // Apply on load\n applyDarkMode();\n \n // Re-apply on dynamic content\n var observer = new MutationObserver(function(mutations) {\n if (enabled && !document.getElementById('universal-dark-mode-style')) {\n applyDarkMode();\n }\n });\n observer.observe(document.head, { childList: true });\n \n console.log('[Universal Dark Mode] Loaded - Press Alt+Shift+D to toggle');\n})();", "Universal Dark Mode"); } } catch(__e) { console.warn('[Userscript:Universal Dark Mode]', __e); } })(); })();
Skip to content

Repository files navigation

seqcluster

Ask questions in the repo's Gitter: Join the chat at:

small RNA analysis from NGS data

https://travis-ci.org/lpantano/seqcluster.png?branch=masterhttps://img.shields.io/badge/install%20with-bioconda-brightgreen.svg?style=flat-squareProject Status: Active - The project has reached a stable, usable state and is being actively developed.https://img.shields.io/badge/DOI-10.1093%2Fbioinformatics%2Fbtv632-lightgrey.svg?style=flat-square

Cite

Specific small-RNA signatures in the amygdala at premotor and motor stages of Parkinson's disease revealed by deep sequencing analysis. Pantano L, Friedländer MR, Escaramís G, Lizano E, Pallarès-Albanell J, Ferrer I, Estivill X, Martí E. Bioinformatics. 2015 Nov 2. pii: btv632. [Epub ahead of print] PMID: 26530722

A non-biased framework for the annotation and classification of the non-miRNA small RNA transcriptome. Pantano L1, Estivill X, Martí E. Bioinformatics. 2011 Nov 15;27(22):3202-3. doi: 10.1093/bioinformatics/btr527. Epub 2011 Oct 5. PMID: 21976421

Quick start links

See installation at http://seqcluster.readthedocs.org/installation.html

Moreover bcbio-nextgen provides a python framework to run a whole pipeline for small RNA (miRNA + tRNA + piRNA + others).

An example of how to run with bcbio is here: http://seqcluster.readthedocs.org/example_pipeline.html#mirqc-data

In case you want to use seqcluster alone, a complete tutorial is here: http://seqcluster.readthedocs.org/getting_started.html#clustering-of-small-rna-sequences

Report

Seqcluster creates html report that looks like this. That is a table of all cluster detected, and you can go into each of them and get a complete description with profile expression figures, annotation details and sequences counts for each sample. An interactive report is as well available to explore the expression profile, position on the genome and secondary structure. Learn more http://seqcluster.readthedocs.org/more_outputs.html#report.

Contributors

  • Lorena Pantano (Bioinformatic Core, Harvard Chan School, Boston, USA)
  • Judith Flo Gaya (School of Engineer and Aplied Science- Harvard University, Boston, USA)
  • Eulalia Marti Puig (Genomics and Disease, Center of Genomic Regulation, Barcelona, Spain)
  • Francisco Pantano Rubino: Architect
  • Steffen Möller (University of Rostock)
Bitdeli badge

About

small RNA analysis from NGS data

Topics

Resources

Stars

39 stars

Watchers

1 watching

Forks

Releases

Packages

Used by

Contributors

Languages