CelloType (Nature Methods 2024) is an end-to-end Transformer-based method for automated cell/nucleus segmentation and cell-type classification.
[Documentation]
- Improved Precision: For both segmentation and classification
- Wide Applicability: Various types of images (fluorescent, brighfield, natural)
- Multi-scale: Capable of classifying diverse cell types and microanatomical structures
Our codes are based on open-source projects Detectron2, Mask DINO.
First, install dependencies
- Linux with Python = 3.8
- Detectron2: follow Detectron2 installation instructions.
# create conda environment
conda create --name cellotype python=3.8
conda activate cellotype
# install pytorch and detectron2
conda install pytorch==1.9.0 torchvision==0.10.0 cudatoolkit=11.1 -c pytorch -c nvidia
python -m pip install 'git+https://github.com/facebookresearch/detectron2.git'# (add --user if you don't have permission)# Compile Deformable-DETR CUDA operators
git clone https://github.com/fundamentalvision/Deformable-DETR.git
cd Deformable-DETR
cd ./models/ops
sh ./make.sh
# clone and install the project
pip install cellotypeClone the repository:
git clone https://github.com/maxpmx/CelloType.git
cd CelloTypeThen Download the model weights:
cd models
sh download.sh
cd ..fromskimageimportiofromcellotype.predictimportCelloTypePredictorimg=io.imread('data/example/example_tissuenet.png') # [H, W, 3]model=CelloTypePredictor(model_path='./models/tissuenet_model_0019999.pth',
confidence_thresh=0.3, max_det=1000, device='cuda', config_path='./configs/maskdino_R50_bs16_50ep_4s_dowsample1_2048.yaml')
mask=model.predict(img) # [H, W]The documentation is available at CelloType
@article{pang2024cellotype,
title={CelloType: A Unified Model for Segmentation and Classification of Tissue Images},
author={Pang, Minxing and Roy, Tarun Kanti and Wu, Xiaodong and Tan, Kai},
journal={Nature Methods},
year={2024}
}
Many thanks to these projects