Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Add copy buttons to all
 blocks
(function() {
function addCopyButtons() {
document.querySelectorAll('pre code').forEach(function(codeBlock) {
if (codeBlock.parentElement.hasAttribute('data-copy-added')) return;
codeBlock.parentElement.setAttribute('data-copy-added', 'true');
var btn = document.createElement('button');
btn.textContent = 'Copy';
btn.style.cssText = 'position:absolute;top:4px;right:4px;padding:2px 8px;font-size:11px;background:#4ecdc4;border:none;border-radius:4px;color:#1a1a2e;cursor:pointer;opacity:0.7;transition:opacity 0.2s;';
btn.onmouseover = function() { this.style.opacity = '1'; };
btn.onmouseout = function() { this.style.opacity = '0.7'; };
btn.onclick = function() {
navigator.clipboard.writeText(codeBlock.textContent).then(function() {
btn.textContent = 'Copied!';
setTimeout(function() { btn.textContent = 'Copy'; }, 1500);
});
};
codeBlock.parentElement.style.position = 'relative';
codeBlock.parentElement.appendChild(btn);
});
}
addCopyButtons();
// Re-run on dynamic content
var observer = new MutationObserver(addCopyButtons);
observer.observe(document.body, { childList: true, subtree: true });
})();
}
} catch(__e) { console.warn('[Userscript:Add Copy Buttons to Code Blocks]', __e); }
})();
(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Force GitHub README to respect dark mode (function() { var style = document.createElement('style'); style.textContent = ' .markdown-body { color-scheme: dark light; } .markdown-body pre { background: #161b22 !important; } .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; } .markdown-body table th, .markdown-body table td { border-color: #30363d !important; } .markdown-body img { background: #0d1117; } .markdown-body blockquote { border-left-color: #8b949e; } .markdown-body hr { border-color: #30363d; } '; document.head.appendChild(style); })(); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Highlight search terms from Google/DuckDuckGo/Bing referrer (function() { var ref = document.referrer; var terms = []; if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) { var url = new URL(ref); var q = url.searchParams.get('q') || url.searchParams.get('p'); if (q) { terms = q.split(/\s+/).filter(function(t) { return t.length > 2; }); } } if (terms.length === 0) return; var style = document.createElement('style'); style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }'; document.head.appendChild(style); function highlight(node) { if (node.nodeType === 3) { // text node var text = node.textContent; var found = false; terms.forEach(function(term) { var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\]\\]/g, '\\') + ')', 'gi'); if (regex.test(text)) { found = true; var frag = document.createDocumentFragment(); var parts = text.split(regex); parts.forEach(function(part, i) { if (i % 2 === 0) { frag.appendChild(document.createTextNode(part)); } else { var span = document.createElement('span'); span.className = 'userscript-highlight'; span.textContent = part; frag.appendChild(span); } }); node.parentNode.replaceChild(frag, node); } }); } else if (node.nodeType === 1 && node.childNodes) { // element var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT']; if (!skipTags.includes(node.tagName)) { Array.from(node.childNodes).forEach(highlight); } } } highlight(document.body); // Re-highlight on dynamic content var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1 || node.nodeType === 3) highlight(node); }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Strip utm_, fbclid, gclid, etc. from all links on page (function() { var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content', 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid', 'ref', 'ref_src', 'source', 'medium', 'campaign']; function cleanUrl(url) { try { var u = new URL(url, window.location.origin); var changed = false; trackingParams.forEach(function(p) { if (u.searchParams.has(p)) { u.searchParams.delete(p); changed = true; } }); return changed ? u.toString() : url; } catch (e) { return url; } } function cleanLinks() { document.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } cleanLinks(); var observer = new MutationObserver(function(mutations) { mutations.forEach(function(m) { m.addedNodes.forEach(function(node) { if (node.nodeType === 1) { if (node.tagName === 'A') cleanLinks(); node.querySelectorAll('a[href]').forEach(function(a) { var clean = cleanUrl(a.href); if (clean !== a.href) a.href = clean; }); } }); }); }); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Auto-enable theater mode on YouTube (function() { function tryTheater() { var btn = document.querySelector('button[aria-label="Theater mode"], ytd-player #player button[title="Theater mode"]'); if (btn && !btn.classList.contains('activated')) { btn.click(); } } // Try immediately tryTheater(); // Try after navigation (SPA) var lastUrl = location.href; setInterval(function() { if (location.href !== lastUrl) { lastUrl = location.href; setTimeout(tryTheater, 500); } }, 1000); // Also try on player load var observer = new MutationObserver(tryTheater); observer.observe(document.body, { childList: true, subtree: true }); })(); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Remove or un-stick sticky/fixed headers that block content (function() { function unstick() { document.querySelectorAll('header, nav, [role="banner"], .header, .navbar, .sticky, .fixed-top, [style*="position: fixed"], [style*="position:sticky"]').forEach(function(el) { if (el.style.position === 'fixed' || el.style.position === 'sticky' || getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') { el.style.position = 'static'; el.style.top = 'auto'; el.style.zIndex = 'auto'; } }); } unstick(); var observer = new MutationObserver(unstick); observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] }); })(); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger
, 'i'); if (__m === '*' || __re.test(location.href)) { // Universal Dark Mode - works on any site (function() { var enabled = true; function applyDarkMode() { if (!enabled) return; // Create style element if it doesn't exist var style = document.getElementById('universal-dark-mode-style'); if (!style) { style = document.createElement('style'); style.id = 'universal-dark-mode-style'; document.head.appendChild(style); } // Dark mode CSS - inverts colors but preserves images/video style.textContent = ' /* Invert everything except media */ html { filter: invert(1) hue-rotate(180deg) !important; background: #1a1a2e !important; } /* Restore images, videos, iframes, canvas */ img, video, iframe, canvas, svg, picture, [style*="background-image"] { filter: invert(1) hue-rotate(180deg) !important; } /* Preserve specific elements that should not be inverted */ .no-dark-mode, .no-dark-mode *, [data-theme="light"], [data-theme="light"], .ace_editor, .ace_editor *, .CodeMirror, .CodeMirror *, .monaco-editor, .monaco-editor *, .markdown-body pre, .markdown-body pre *, .highlight, .highlight *, pre code, pre code * { filter: none !important; } /* Fix common UI elements */ .modal, .popup, .dropdown-menu, .tooltip, .popover { filter: invert(1) hue-rotate(180deg) !important; background: #2d2d44 !important; border-color: #444 !important; } /* Scrollbars */ ::-webkit-scrollbar { background: #1a1a2e !important; } ::-webkit-scrollbar-thumb { background: #444 !important; } ::-webkit-scrollbar-thumb:hover { background: #555 !important; } /* Selection */ ::selection { background: #4ecdc4 !important; color: #1a1a2e !important; } ::-moz-selection { background: #4ecdc4 !important; color: #1a1a2e !important; } '; } function removeDarkMode() { var style = document.getElementById('universal-dark-mode-style'); if (style) style.remove(); } // Toggle with Alt+Shift+D document.addEventListener('keydown', function(e) { if (e.altKey && e.shiftKey && e.key === 'D') { e.preventDefault(); enabled = !enabled; if (enabled) { applyDarkMode(); console.log('[Universal Dark Mode] Enabled'); } else { removeDarkMode(); console.log('[Universal Dark Mode] Disabled'); } } }); // Apply on load applyDarkMode(); // Re-apply on dynamic content var observer = new MutationObserver(function(mutations) { if (enabled && !document.getElementById('universal-dark-mode-style')) { applyDarkMode(); } }); observer.observe(document.head, { childList: true }); console.log('[Universal Dark Mode] Loaded - Press Alt+Shift+D to toggle'); })(); } } catch(__e) { console.warn('[Userscript:Universal Dark Mode]', __e); } })(); })();
Skip to content

Support for NuGen Ovation RRBS libraries - #379

Open
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation
Open

Support for NuGen Ovation RRBS libraries#379
jma1991 wants to merge 3 commits into
nf-core:devfrom
zifornd:nugen-ovation

Conversation

@jma1991

Copy link
Copy Markdown

This pull request introduces an initial implementation for processing NuGen Ovation RRBS libraries, drawing guidance from the commands outlined in the NuMetRRBS repository. Currently, the focus is on integrating fundamental steps such as adapter trimming and diversity trimming. Although there's an optional component for deduplicating reads via molecular tags, I'm not sure how to implement this right now. Lastly, let me know if I should submit some Ovation data to the test-datasets repository.

PR checklist

  • This comment contains a description of changes (with reason).
  • If you've fixed a bug or added code that should be tested, add tests!
  • If you've added a new tool - have you followed the pipeline conventions in the contribution docs
  • If necessary, also make a PR on the nf-core/methylseq branch on the nf-core/test-datasets repository.
  • Make sure your code lints (nf-core lint).
  • Ensure the test suite passes (nextflow run . -profile test,docker --outdir <OUTDIR>).
  • Check for unexpected warnings in debug mode (nextflow run . -profile debug,test,docker --outdir <OUTDIR>).
  • Usage Documentation in docs/usage.md is updated.
  • Output Documentation in docs/output.md is updated.
  • CHANGELOG.md is updated.
  • README.md is updated (including new tool citations and authors/contributors).

@jma1991
jma1991 requested a review from a team as a code ownerFebruary 14, 2024 14:53

@FelixKruegerFelixKrueger left a comment

Copy link
Copy Markdown
Contributor

Choose a reason for hiding this comment

The reason will be displayed to describe this comment to others. Learn more.

The suggested diversity trimming looks good to me, but do we have specific test data for this?

@jma1991

Copy link
Copy Markdown
Author

The suggested diversity trimming looks good to me, but do we have specific test data for this?

I have some Ovation test data, shall I submit it to the test-datasets repository?

@ewels

Copy link
Copy Markdown
Member

@jma1991 could you please update / rebase this PR off the latest nf-core dev branch please?

@sateeshperi

Copy link
Copy Markdown
Contributor

@jma1991 checking in here - did you get some test data into the test-datasets repo ?

@sateeshperisateeshperi added the WIP Work in progress label Sep 23, 2024
@sateeshperisateeshperi added the stale Will eventually be closed due to divergence and compatibility issues. label Aug 13, 2025
Sign up for freeto join this conversation on GitHub. Already have an account? Sign in to comment

Labels

staleWill eventually be closed due to divergence and compatibility issues.WIPWork in progress

Projects

None yet

Development

Successfully merging this pull request may close these issues.

4 participants

@jma1991@ewels@sateeshperi@FelixKrueger