Hi, I have been trying to use Filtlong on some long read assemblers, mainly Flye and Raven. I have used it with Unicycler for a while now with no issues, however, there seems to be something with the formatting of the output file that is not accepted by Flye, Raven, or by Fastqc. When running through Fastqc it returns SequenceFormatException: Midline ' read sequence ' didnt start with '+'. I have also attached a link to a BioStars post I made a few weeks ago detailing what I was seeing when opening the fastq and going to the specified line. I've run this with different fastqs, some that were sequenced in house and some by others, so I don't think the original fastqs are an issue.
Thank you!
Hi, I have been trying to use Filtlong on some long read assemblers, mainly Flye and Raven. I have used it with Unicycler for a while now with no issues, however, there seems to be something with the formatting of the output file that is not accepted by Flye, Raven, or by Fastqc. When running through Fastqc it returns SequenceFormatException: Midline ' read sequence ' didnt start with '+'. I have also attached a link to a BioStars post I made a few weeks ago detailing what I was seeing when opening the fastq and going to the specified line. I've run this with different fastqs, some that were sequenced in house and some by others, so I don't think the original fastqs are an issue.
Thank you!