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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Add copy buttons to all
 blocks\n(function() {\n function addCopyButtons() {\n document.querySelectorAll('pre code').forEach(function(codeBlock) {\n if (codeBlock.parentElement.hasAttribute('data-copy-added')) return;\n codeBlock.parentElement.setAttribute('data-copy-added', 'true');\n \n var btn = document.createElement('button');\n btn.textContent = 'Copy';\n btn.style.cssText = 'position:absolute;top:4px;right:4px;padding:2px 8px;font-size:11px;background:#4ecdc4;border:none;border-radius:4px;color:#1a1a2e;cursor:pointer;opacity:0.7;transition:opacity 0.2s;';\n btn.onmouseover = function() { this.style.opacity = '1'; };\n btn.onmouseout = function() { this.style.opacity = '0.7'; };\n btn.onclick = function() {\n navigator.clipboard.writeText(codeBlock.textContent).then(function() {\n btn.textContent = 'Copied!';\n setTimeout(function() { btn.textContent = 'Copy'; }, 1500);\n });\n };\n codeBlock.parentElement.style.position = 'relative';\n codeBlock.parentElement.appendChild(btn);\n });\n }\n \n addCopyButtons();\n \n // Re-run on dynamic content\n var observer = new MutationObserver(addCopyButtons);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Add Copy Buttons to Code Blocks");
}
} catch(__e) { console.warn('[Userscript:Add Copy Buttons to Code Blocks]', __e); }
})();
(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Force GitHub README to respect dark mode\n(function() {\n var style = document.createElement('style');\n style.textContent = '\n .markdown-body {\n color-scheme: dark light;\n }\n .markdown-body pre { background: #161b22 !important; }\n .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; }\n .markdown-body table th, .markdown-body table td { border-color: #30363d !important; }\n .markdown-body img { background: #0d1117; }\n .markdown-body blockquote { border-left-color: #8b949e; }\n .markdown-body hr { border-color: #30363d; }\n ';\n document.head.appendChild(style);\n})();", "GitHub Dark Mode README Fix"); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Highlight search terms from Google/DuckDuckGo/Bing referrer\n(function() {\n var ref = document.referrer;\n var terms = [];\n \n if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) {\n var url = new URL(ref);\n var q = url.searchParams.get('q') || url.searchParams.get('p');\n if (q) {\n terms = q.split(/\\s+/).filter(function(t) { return t.length > 2; });\n }\n }\n \n if (terms.length === 0) return;\n \n var style = document.createElement('style');\n style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }';\n document.head.appendChild(style);\n \n function highlight(node) {\n if (node.nodeType === 3) { // text node\n var text = node.textContent;\n var found = false;\n terms.forEach(function(term) {\n var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\\]\\\\]/g, '\\\\') + ')', 'gi');\n if (regex.test(text)) {\n found = true;\n var frag = document.createDocumentFragment();\n var parts = text.split(regex);\n parts.forEach(function(part, i) {\n if (i % 2 === 0) {\n frag.appendChild(document.createTextNode(part));\n } else {\n var span = document.createElement('span');\n span.className = 'userscript-highlight';\n span.textContent = part;\n frag.appendChild(span);\n }\n });\n node.parentNode.replaceChild(frag, node);\n }\n });\n } else if (node.nodeType === 1 && node.childNodes) { // element\n var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT'];\n if (!skipTags.includes(node.tagName)) {\n Array.from(node.childNodes).forEach(highlight);\n }\n }\n }\n \n highlight(document.body);\n \n // Re-highlight on dynamic content\n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1 || node.nodeType === 3) highlight(node);\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Highlight Search Terms"); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Strip utm_, fbclid, gclid, etc. from all links on page\n(function() {\n var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content',\n 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid',\n 'ref', 'ref_src', 'source', 'medium', 'campaign'];\n \n function cleanUrl(url) {\n try {\n var u = new URL(url, window.location.origin);\n var changed = false;\n trackingParams.forEach(function(p) {\n if (u.searchParams.has(p)) {\n u.searchParams.delete(p);\n changed = true;\n }\n });\n return changed ? u.toString() : url;\n } catch (e) {\n return url;\n }\n }\n \n function cleanLinks() {\n document.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n \n cleanLinks();\n \n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1) {\n if (node.tagName === 'A') cleanLinks();\n node.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Remove Tracking Parameters from Links"); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Auto-enable theater mode on YouTube\n(function() {\n function tryTheater() {\n var btn = document.querySelector('button[aria-label=\"Theater mode\"], ytd-player #player button[title=\"Theater mode\"]');\n if (btn && !btn.classList.contains('activated')) {\n btn.click();\n }\n }\n \n // Try immediately\n tryTheater();\n \n // Try after navigation (SPA)\n var lastUrl = location.href;\n setInterval(function() {\n if (location.href !== lastUrl) {\n lastUrl = location.href;\n setTimeout(tryTheater, 500);\n }\n }, 1000);\n \n // Also try on player load\n var observer = new MutationObserver(tryTheater);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "YouTube Theater Mode Default"); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Remove or un-stick sticky/fixed headers that block content\n(function() {\n function unstick() {\n document.querySelectorAll('header, nav, [role=\"banner\"], .header, .navbar, .sticky, .fixed-top, [style*=\"position: fixed\"], [style*=\"position:sticky\"]').forEach(function(el) {\n if (el.style.position === 'fixed' || el.style.position === 'sticky' || \n getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') {\n el.style.position = 'static';\n el.style.top = 'auto';\n el.style.zIndex = 'auto';\n }\n });\n }\n \n unstick();\n \n var observer = new MutationObserver(unstick);\n observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] });\n})();", "Kill Sticky Headers"); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,60 @@
"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,31 @@
"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,24 @@
"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,4 @@
Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,14 @@
"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
Original file line numberDiff line numberDiff line change
Expand Up@@ -4,8 +4,9 @@

```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
Loading
Loading
, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Universal Dark Mode - works on any site\n(function() {\n var enabled = true;\n \n function applyDarkMode() {\n if (!enabled) return;\n \n // Create style element if it doesn't exist\n var style = document.getElementById('universal-dark-mode-style');\n if (!style) {\n style = document.createElement('style');\n style.id = 'universal-dark-mode-style';\n document.head.appendChild(style);\n }\n \n // Dark mode CSS - inverts colors but preserves images/video\n style.textContent = '\n /* Invert everything except media */\n html {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #1a1a2e !important;\n }\n \n /* Restore images, videos, iframes, canvas */\n img, video, iframe, canvas, svg, picture, [style*=\"background-image\"] {\n filter: invert(1) hue-rotate(180deg) !important;\n }\n \n /* Preserve specific elements that should not be inverted */\n .no-dark-mode, .no-dark-mode *,\n [data-theme=\"light\"], [data-theme=\"light\"],\n .ace_editor, .ace_editor *,\n .CodeMirror, .CodeMirror *,\n .monaco-editor, .monaco-editor *,\n .markdown-body pre, .markdown-body pre *,\n .highlight, .highlight *,\n pre code, pre code * {\n filter: none !important;\n }\n \n /* Fix common UI elements */\n .modal, .popup, .dropdown-menu, .tooltip, .popover {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #2d2d44 !important;\n border-color: #444 !important;\n }\n \n /* Scrollbars */\n ::-webkit-scrollbar { background: #1a1a2e !important; }\n ::-webkit-scrollbar-thumb { background: #444 !important; }\n ::-webkit-scrollbar-thumb:hover { background: #555 !important; }\n \n /* Selection */\n ::selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ::-moz-selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ';\n }\n \n function removeDarkMode() {\n var style = document.getElementById('universal-dark-mode-style');\n if (style) style.remove();\n }\n \n // Toggle with Alt+Shift+D\n document.addEventListener('keydown', function(e) {\n if (e.altKey && e.shiftKey && e.key === 'D') {\n e.preventDefault();\n enabled = !enabled;\n if (enabled) {\n applyDarkMode();\n console.log('[Universal Dark Mode] Enabled');\n } else {\n removeDarkMode();\n console.log('[Universal Dark Mode] Disabled');\n }\n }\n });\n \n // Apply on load\n applyDarkMode();\n \n // Re-apply on dynamic content\n var observer = new MutationObserver(function(mutations) {\n if (enabled && !document.getElementById('universal-dark-mode-style')) {\n applyDarkMode();\n }\n });\n observer.observe(document.head, { childList: true });\n \n console.log('[Universal Dark Mode] Loaded - Press Alt+Shift+D to toggle');\n})();", "Universal Dark Mode"); } } catch(__e) { console.warn('[Userscript:Universal Dark Mode]', __e); } })(); })();
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4 changes: 4 additions & 0 deletions .gitignore
Original file line numberDiff line numberDiff line change
Expand Up@@ -22,6 +22,10 @@ __pycache__/
# docs
/docs/generated/
/docs/_build/
/docs/auto_gallery/
/docs/sg_execution_times.rst
/docs/gallery/**/data/
/docs/gallery/**/__pycache__/

# IDEs
/.idea/
Expand Down
5 changes: 5 additions & 0 deletions docs/_static/css/custom.css
Original file line numberDiff line numberDiff line change
Expand Up@@ -2,3 +2,8 @@
div.cell_output table.dataframe {
font-size: 0.8em;
}

/* Gallery landing: subsection links are shown in the sidebar; hide the duplicate inline list. */
section#gallery > .toctree-wrapper {
display: none;
}
20 changes: 20 additions & 0 deletions docs/conf.py
Original file line numberDiff line numberDiff line change
Expand Up@@ -56,6 +56,7 @@
"sphinx.ext.intersphinx",
"sphinx.ext.autosummary",
"sphinx.ext.napoleon",
"sphinx_gallery.gen_gallery",
"sphinxcontrib.bibtex",
"sphinxcontrib.katex",
"sphinx_autodoc_typehints",
Expand DownExpand Up@@ -118,6 +119,9 @@
"tutorials/notebooks/README.md",
"tutorials/notebooks/references.md",
"tutorials/notebooks/notebooks/paper_reproducibility/*",
"gallery/*",
"auto_gallery/**/*.ipynb",
"auto_gallery/**/*.py",
]


Expand DownExpand Up@@ -157,3 +161,19 @@
# you can add an exception to this list.
("py:class", "igraph.Graph"),
]

# -- Sphinx-Gallery configuration -------------------------------------------

sys.path.insert(0, str(HERE / "gallery"))

sphinx_gallery_conf = {
"examples_dirs": ["gallery"],
"gallery_dirs": ["auto_gallery"],
"filename_pattern": r"/plot_",
"ignore_pattern": r"(__init__|_helpers)\.py",
"image_scrapers": ("matplotlib",),
"matplotlib_animations": True,
"within_subsection_order": "FileNameSortKey",
"nested_sections": True,
"download_all_examples": True,
}
15 changes: 15 additions & 0 deletions docs/gallery.rst
Original file line numberDiff line numberDiff line change
@@ -0,0 +1,15 @@
Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.

.. toctree::
:maxdepth: 1

auto_gallery/basic/index
auto_gallery/customization/index
auto_gallery/overlays/index

.. include:: auto_gallery/index.rst
:start-after: Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
:end-before: .. toctree::
4 changes: 4 additions & 0 deletions docs/gallery/README.rst
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Gallery
-------

Examples demonstrating the plotting capabilities of ``spatialdata-plot``.
60 changes: 60 additions & 0 deletions docs/gallery/_helpers.py
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"""Shared data loaders for gallery examples."""

from __future__ import annotations

import warnings

import numpy as np
import scanpy as sc
import spatialdata as sd
from spatialdata.models import Image2DModel, ShapesModel, TableModel


def load_visium_breast_cancer() -> sd.SpatialData:
"""Load Visium breast cancer tissue as a SpatialData object.

Uses ``scanpy.datasets.visium_sge`` which caches the download
via :mod:`pooch`, so the data is only fetched once.
"""
with warnings.catch_warnings():
warnings.simplefilter("ignore")
adata = sc.datasets.visium_sge(
sample_id="V1_Breast_Cancer_Block_A_Section_1",
)

sample = list(adata.uns["spatial"].keys())[0]
meta = adata.uns["spatial"][sample]
sf = meta["scalefactors"]["tissue_hires_scalef"]

image = Image2DModel.parse(
np.moveaxis(meta["images"]["hires"], -1, 0),
dims=("c", "y", "x"),
)

radius = meta["scalefactors"]["spot_diameter_fullres"] * sf / 2
circles = ShapesModel.parse(
adata.obsm["spatial"] * sf,
geometry=0,
radius=radius,
index=adata.obs_names,
)

adata.obs["region"] = "spots"
adata.obs["region"] = adata.obs["region"].astype("category")
adata.obs["instance_key"] = adata.obs_names
table = TableModel.parse(
adata,
region="spots",
region_key="region",
instance_key="instance_key",
)

table.var_names_make_unique()
sc.pp.normalize_total(table)
sc.pp.log1p(table)

return sd.SpatialData(
images={"tissue": image},
shapes={"spots": circles},
tables={"table": table},
)
4 changes: 4 additions & 0 deletions docs/gallery/basic/README.rst
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Basic
-----

Rendering individual spatial element types.
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_images.py
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"""
Render tissue image
===================

Render an H&E tissue image from a Visium experiment.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels.py
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"""
Render labels
=============

Render a cell segmentation mask.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels").pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_labels_contour.py
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"""
Render label contours
=====================

Render segmentation boundaries using ``contour_px``.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_labels("blobs_labels", contour_px=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_points.py
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"""
Render points
=============

Render transcript detections as points.
"""

import spatialdata as sd

import spatialdata_plot # noqa: F401

sdata = sd.datasets.blobs()

sdata.pl.render_points("blobs_points", size=3).pl.show()
14 changes: 14 additions & 0 deletions docs/gallery/basic/plot_render_shapes.py
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"""
Render spots
============

Render Visium spot shapes on their own.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_shapes("spots").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/customization/README.rst
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Customization
-------------

Styling options: colormaps, outlines, contours, and alpha.
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_gene_cmap.py
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"""
Gene expression with custom colormap
=====================================

Color spots by gene expression using a custom colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", cmap="magma", fill_alpha=0.8).pl.show())
31 changes: 31 additions & 0 deletions docs/gallery/customization/plot_multi_panel.py
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"""
Multi-panel layout
==================

Display multiple coordinate systems side by side.
"""

import numpy as np
import spatialdata as sd
from spatialdata.models import Image2DModel
from spatialdata.transformations import Identity

import spatialdata_plot # noqa: F401

rng = np.random.default_rng(0)
img_a = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_a": Identity()},
)
img_b = Image2DModel.parse(
rng.random((3, 64, 64)),
dims=("c", "y", "x"),
transformations={"sample_b": Identity()},
)
sdata = sd.SpatialData(images={"img_a": img_a, "img_b": img_b})

sdata.pl.render_images().pl.show(
coordinate_systems=["sample_a", "sample_b"],
figsize=(8, 4),
)
24 changes: 24 additions & 0 deletions docs/gallery/customization/plot_shapes_outline.py
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"""
Spots with outlines
===================

Style spots with visible outlines and translucent fill.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(
sdata.pl.render_images("tissue")
.pl.render_shapes(
"spots",
fill_alpha=0.3,
outline_width=1.5,
outline_color="black",
outline_alpha=1.0,
)
.pl.show()
)
14 changes: 14 additions & 0 deletions docs/gallery/customization/plot_single_channel.py
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"""
Single channel with colormap
=============================

Render one image channel with a colormap.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

sdata.pl.render_images("tissue", channel=0, cmap="viridis").pl.show()
4 changes: 4 additions & 0 deletions docs/gallery/overlays/README.rst
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Overlays
--------

Combining multiple spatial element layers in a single plot.
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_category.py
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"""
Color spots by category
=======================

Overlay spots colored by a categorical annotation.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_color_by_gene.py
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"""
Color spots by gene expression
===============================

Overlay spots colored by a gene on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="ERBB2", fill_alpha=0.8).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_filter_groups.py
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"""
Filter by category groups
=========================

Show only selected categories using the ``groups`` parameter.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", color="in_tissue", groups=["1"], fill_alpha=0.7).pl.show())
14 changes: 14 additions & 0 deletions docs/gallery/overlays/plot_images_shapes.py
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"""
Tissue with spots
=================

Overlay Visium spots on an H&E tissue image.
"""

from _helpers import load_visium_breast_cancer

import spatialdata_plot # noqa: F401

sdata = load_visium_breast_cancer()

(sdata.pl.render_images("tissue").pl.render_shapes("spots", fill_alpha=0.5).pl.show())
3 changes: 2 additions & 1 deletion docs/index.md
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```{toctree}
:hidden: true
:maxdepth: 1
:maxdepth: 2

gallery
api.md
changelog.md
contributing.md
Expand Down
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