Hey, I downloaded the latest lambda (1.9.4) and rebuilt my indexes. However, now I get the problem that all these indexes are not useable:
LAMBDA - the Local Aligner for Massive Biological DatA
Version 1.9.4
Reading index properties...
ERROR: The following exception was thrown while reading the index:
"Your lambda executable was built with LAMBDA_LONG_PROTEIN_SUBJ_SEQS, but the index was created by an executable that was built without it.You need to recreate the index or rebuild Lambda."
Make sure the directory exists and is readable; recreate the index and try again.
If the problem persists, report an issue at https://github.com/seqan/lambda/issues and include this output, as well as the output of lambda2 --version, thanks!
system call
/g/bork3/home/hildebra/dev/Perl/reAssemble2Spec//bin/lambda2-1.9.4-Linux-x86_64/bin/./lambda2 searchn -t 24 --percent-identity 75 -n 200 -e 1e-12 --seed-offset 7 --seed-length 14 --seed-delta 1 -b -3 --filter-putative-duplicates on -q /g/scb/bork/hildebra/Tamoc/GCglobal/GGC13001/ribos/reads_SSU.fna -i /g/bork3/home/hildebra/DB/MarkerG/PR2//gb203_pr2_all_10_28_99p.fasta.lambda -o /MF//GGC13001ITS//LCA/SSUriboRun_bl.0.m8.gz
The index was built using this command:
/g/bork3/home/hildebra/dev/Perl/reAssemble2Spec//bin/lambda2-1.9.4-Linux-x86_64/bin/./lambda2 mkindexn -t 16 -d /g/bork3/home/hildebra/DB/MarkerG/PR2//gb203_pr2_all_10_28_99p.fasta
How can I fix this? I'm not even using a protein DB.
many thanks,
Falk
Hey, I downloaded the latest lambda (1.9.4) and rebuilt my indexes. However, now I get the problem that all these indexes are not useable:
LAMBDA - the Local Aligner for Massive Biological DatA
Version 1.9.4
Reading index properties...
ERROR: The following exception was thrown while reading the index:
"Your lambda executable was built with LAMBDA_LONG_PROTEIN_SUBJ_SEQS, but the index was created by an executable that was built without it.You need to recreate the index or rebuild Lambda."
Make sure the directory exists and is readable; recreate the index and try again.
If the problem persists, report an issue at https://github.com/seqan/lambda/issues and include this output, as well as the output of
lambda2 --version, thanks!system call
/g/bork3/home/hildebra/dev/Perl/reAssemble2Spec//bin/lambda2-1.9.4-Linux-x86_64/bin/./lambda2 searchn -t 24 --percent-identity 75 -n 200 -e 1e-12 --seed-offset 7 --seed-length 14 --seed-delta 1 -b -3 --filter-putative-duplicates on -q /g/scb/bork/hildebra/Tamoc/GCglobal/GGC13001/ribos/reads_SSU.fna -i /g/bork3/home/hildebra/DB/MarkerG/PR2//gb203_pr2_all_10_28_99p.fasta.lambda -o /MF//GGC13001ITS//LCA/SSUriboRun_bl.0.m8.gz
The index was built using this command:
/g/bork3/home/hildebra/dev/Perl/reAssemble2Spec//bin/lambda2-1.9.4-Linux-x86_64/bin/./lambda2 mkindexn -t 16 -d /g/bork3/home/hildebra/DB/MarkerG/PR2//gb203_pr2_all_10_28_99p.fasta
How can I fix this? I'm not even using a protein DB.
many thanks,
Falk