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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Add copy buttons to all
 blocks\n(function() {\n function addCopyButtons() {\n document.querySelectorAll('pre code').forEach(function(codeBlock) {\n if (codeBlock.parentElement.hasAttribute('data-copy-added')) return;\n codeBlock.parentElement.setAttribute('data-copy-added', 'true');\n \n var btn = document.createElement('button');\n btn.textContent = 'Copy';\n btn.style.cssText = 'position:absolute;top:4px;right:4px;padding:2px 8px;font-size:11px;background:#4ecdc4;border:none;border-radius:4px;color:#1a1a2e;cursor:pointer;opacity:0.7;transition:opacity 0.2s;';\n btn.onmouseover = function() { this.style.opacity = '1'; };\n btn.onmouseout = function() { this.style.opacity = '0.7'; };\n btn.onclick = function() {\n navigator.clipboard.writeText(codeBlock.textContent).then(function() {\n btn.textContent = 'Copied!';\n setTimeout(function() { btn.textContent = 'Copy'; }, 1500);\n });\n };\n codeBlock.parentElement.style.position = 'relative';\n codeBlock.parentElement.appendChild(btn);\n });\n }\n \n addCopyButtons();\n \n // Re-run on dynamic content\n var observer = new MutationObserver(addCopyButtons);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Add Copy Buttons to Code Blocks");
}
} catch(__e) { console.warn('[Userscript:Add Copy Buttons to Code Blocks]', __e); }
})();
(function(){
try {
var __m = "github.com";
var __re = new RegExp('^' + "github\\.com" + '
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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Force GitHub README to respect dark mode\n(function() {\n var style = document.createElement('style');\n style.textContent = '\n .markdown-body {\n color-scheme: dark light;\n }\n .markdown-body pre { background: #161b22 !important; }\n .markdown-body code { background: rgba(110, 118, 129, 0.4) !important; }\n .markdown-body table th, .markdown-body table td { border-color: #30363d !important; }\n .markdown-body img { background: #0d1117; }\n .markdown-body blockquote { border-left-color: #8b949e; }\n .markdown-body hr { border-color: #30363d; }\n ';\n document.head.appendChild(style);\n})();", "GitHub Dark Mode README Fix"); } } catch(__e) { console.warn('[Userscript:GitHub Dark Mode README Fix]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

Latest commit

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254 Commits

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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Highlight search terms from Google/DuckDuckGo/Bing referrer\n(function() {\n var ref = document.referrer;\n var terms = [];\n \n if (ref.includes('google.com') || ref.includes('duckduckgo.com') || ref.includes('bing.com')) {\n var url = new URL(ref);\n var q = url.searchParams.get('q') || url.searchParams.get('p');\n if (q) {\n terms = q.split(/\\s+/).filter(function(t) { return t.length > 2; });\n }\n }\n \n if (terms.length === 0) return;\n \n var style = document.createElement('style');\n style.textContent = '.userscript-highlight { background: #fbbf24; color: #1a1a2e; padding: 1px 3px; border-radius: 2px; }';\n document.head.appendChild(style);\n \n function highlight(node) {\n if (node.nodeType === 3) { // text node\n var text = node.textContent;\n var found = false;\n terms.forEach(function(term) {\n var regex = new RegExp('(' + term.replace(/[.*+?^${}()|[\\]\\\\]/g, '\\\\') + ')', 'gi');\n if (regex.test(text)) {\n found = true;\n var frag = document.createDocumentFragment();\n var parts = text.split(regex);\n parts.forEach(function(part, i) {\n if (i % 2 === 0) {\n frag.appendChild(document.createTextNode(part));\n } else {\n var span = document.createElement('span');\n span.className = 'userscript-highlight';\n span.textContent = part;\n frag.appendChild(span);\n }\n });\n node.parentNode.replaceChild(frag, node);\n }\n });\n } else if (node.nodeType === 1 && node.childNodes) { // element\n var skipTags = ['SCRIPT', 'STYLE', 'NOSCRIPT', 'TEXTAREA', 'INPUT', 'SELECT'];\n if (!skipTags.includes(node.tagName)) {\n Array.from(node.childNodes).forEach(highlight);\n }\n }\n }\n \n highlight(document.body);\n \n // Re-highlight on dynamic content\n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1 || node.nodeType === 3) highlight(node);\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Highlight Search Terms"); } } catch(__e) { console.warn('[Userscript:Highlight Search Terms]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Strip utm_, fbclid, gclid, etc. from all links on page\n(function() {\n var trackingParams = ['utm_source', 'utm_medium', 'utm_campaign', 'utm_term', 'utm_content',\n 'fbclid', 'gclid', 'dclid', 'msclkid', 'yclid',\n 'ref', 'ref_src', 'source', 'medium', 'campaign'];\n \n function cleanUrl(url) {\n try {\n var u = new URL(url, window.location.origin);\n var changed = false;\n trackingParams.forEach(function(p) {\n if (u.searchParams.has(p)) {\n u.searchParams.delete(p);\n changed = true;\n }\n });\n return changed ? u.toString() : url;\n } catch (e) {\n return url;\n }\n }\n \n function cleanLinks() {\n document.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n \n cleanLinks();\n \n var observer = new MutationObserver(function(mutations) {\n mutations.forEach(function(m) {\n m.addedNodes.forEach(function(node) {\n if (node.nodeType === 1) {\n if (node.tagName === 'A') cleanLinks();\n node.querySelectorAll('a[href]').forEach(function(a) {\n var clean = cleanUrl(a.href);\n if (clean !== a.href) a.href = clean;\n });\n }\n });\n });\n });\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "Remove Tracking Parameters from Links"); } } catch(__e) { console.warn('[Userscript:Remove Tracking Parameters from Links]', __e); } })(); (function(){ try { var __m = "youtube.com"; var __re = new RegExp('^' + "youtube\\.com" + '
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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages

, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Auto-enable theater mode on YouTube\n(function() {\n function tryTheater() {\n var btn = document.querySelector('button[aria-label=\"Theater mode\"], ytd-player #player button[title=\"Theater mode\"]');\n if (btn && !btn.classList.contains('activated')) {\n btn.click();\n }\n }\n \n // Try immediately\n tryTheater();\n \n // Try after navigation (SPA)\n var lastUrl = location.href;\n setInterval(function() {\n if (location.href !== lastUrl) {\n lastUrl = location.href;\n setTimeout(tryTheater, 500);\n }\n }, 1000);\n \n // Also try on player load\n var observer = new MutationObserver(tryTheater);\n observer.observe(document.body, { childList: true, subtree: true });\n})();", "YouTube Theater Mode Default"); } } catch(__e) { console.warn('[Userscript:YouTube Theater Mode Default]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
Skip to content

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254 Commits

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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

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, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Remove or un-stick sticky/fixed headers that block content\n(function() {\n function unstick() {\n document.querySelectorAll('header, nav, [role=\"banner\"], .header, .navbar, .sticky, .fixed-top, [style*=\"position: fixed\"], [style*=\"position:sticky\"]').forEach(function(el) {\n if (el.style.position === 'fixed' || el.style.position === 'sticky' || \n getComputedStyle(el).position === 'fixed' || getComputedStyle(el).position === 'sticky') {\n el.style.position = 'static';\n el.style.top = 'auto';\n el.style.zIndex = 'auto';\n }\n });\n }\n \n unstick();\n \n var observer = new MutationObserver(unstick);\n observer.observe(document.body, { childList: true, subtree: true, attributes: true, attributeFilter: ['style', 'class'] });\n})();", "Kill Sticky Headers"); } } catch(__e) { console.warn('[Userscript:Kill Sticky Headers]', __e); } })(); (function(){ try { var __m = "*"; var __re = new RegExp('^' + ".*" + '
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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

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, 'i'); if (__m === '*' || __re.test(location.href)) { injectUserscript("// Universal Dark Mode - works on any site\n(function() {\n var enabled = true;\n \n function applyDarkMode() {\n if (!enabled) return;\n \n // Create style element if it doesn't exist\n var style = document.getElementById('universal-dark-mode-style');\n if (!style) {\n style = document.createElement('style');\n style.id = 'universal-dark-mode-style';\n document.head.appendChild(style);\n }\n \n // Dark mode CSS - inverts colors but preserves images/video\n style.textContent = '\n /* Invert everything except media */\n html {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #1a1a2e !important;\n }\n \n /* Restore images, videos, iframes, canvas */\n img, video, iframe, canvas, svg, picture, [style*=\"background-image\"] {\n filter: invert(1) hue-rotate(180deg) !important;\n }\n \n /* Preserve specific elements that should not be inverted */\n .no-dark-mode, .no-dark-mode *,\n [data-theme=\"light\"], [data-theme=\"light\"],\n .ace_editor, .ace_editor *,\n .CodeMirror, .CodeMirror *,\n .monaco-editor, .monaco-editor *,\n .markdown-body pre, .markdown-body pre *,\n .highlight, .highlight *,\n pre code, pre code * {\n filter: none !important;\n }\n \n /* Fix common UI elements */\n .modal, .popup, .dropdown-menu, .tooltip, .popover {\n filter: invert(1) hue-rotate(180deg) !important;\n background: #2d2d44 !important;\n border-color: #444 !important;\n }\n \n /* Scrollbars */\n ::-webkit-scrollbar { background: #1a1a2e !important; }\n ::-webkit-scrollbar-thumb { background: #444 !important; }\n ::-webkit-scrollbar-thumb:hover { background: #555 !important; }\n \n /* Selection */\n ::selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ::-moz-selection { background: #4ecdc4 !important; color: #1a1a2e !important; }\n ';\n }\n \n function removeDarkMode() {\n var style = document.getElementById('universal-dark-mode-style');\n if (style) style.remove();\n }\n \n // Toggle with Alt+Shift+D\n document.addEventListener('keydown', function(e) {\n if (e.altKey && e.shiftKey && e.key === 'D') {\n e.preventDefault();\n enabled = !enabled;\n if (enabled) {\n applyDarkMode();\n console.log('[Universal Dark Mode] Enabled');\n } else {\n removeDarkMode();\n console.log('[Universal Dark Mode] Disabled');\n }\n }\n });\n \n // Apply on load\n applyDarkMode();\n \n // Re-apply on dynamic content\n var observer = new MutationObserver(function(mutations) {\n if (enabled && !document.getElementById('universal-dark-mode-style')) {\n applyDarkMode();\n }\n });\n observer.observe(document.head, { childList: true });\n \n console.log('[Universal Dark Mode] Loaded - Press Alt+Shift+D to toggle');\n})();", "Universal Dark Mode"); } } catch(__e) { console.warn('[Userscript:Universal Dark Mode]', __e); } })(); })();
Skip to content

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254 Commits

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Spatial Omics Summer School

SpatialData
plus
Harpy


This repository contains course material for the Spatial Omics Summer School. The notebooks guide participants through working with SpatialData objects, targeted transcriptomics data and spatial proteomics data.

The tutorials use Harpy for spatial omics analysis workflows and napari-harpy for interactive inspection and visualization in napari.

Installation

This project uses uv to manage the Python environment.

Dependencies are defined in pyproject.toml and locked in uv.lock. Use the lockfile for the course environment so everyone gets the same package versions.

1. Get the repository

Clone this repository and move into the project directory:

git clone https://github.com/vibspatial/targeted_transcriptomics_training.git
cd targeted_transcriptomics_training

If you already cloned the repository, update it from inside the project directory:

git pull

2. Install uv

Make sure uv is installed and available on your PATH.

Check with:

uv --version

3. Create or sync the environment

From the repository root, create or sync the Python 3.12 course environment by running:

uv sync --python 3.12 --locked

This creates or updates the project environment at:

.venv

This means:

  • .venv is created if it does not exist
  • packages are installed from uv.lock
  • uv.lock is not modified
  • the command fails if pyproject.toml and uv.lock are out of sync

4. Activate the environment

On macOS, Linux, or WSL:

source .venv/bin/activate

On Windows PowerShell:

Set-ExecutionPolicy-Scope Process-ExecutionPolicy Bypass
. .\.venv\Scripts\Activate.ps1

On Windows Command Prompt:

.venv\Scripts\activate.bat

On Windows Git Bash:

source .venv/Scripts/activate

5. Download the course data

After activating the environment, download the datasets and model weights used in the notebooks.

On macOS, Linux, or WSL, run:

python download_data.py

On Windows, always set the cache directory explicitly to C:/hp_cache:

python download_data.py --cache-dir-path C:/hp_cache

Use a short cache path on Windows to avoid path-length errors when downloading and unpacking nested dataset files. Some Windows setups still enforce the traditional 260-character path limit unless long-path support is enabled.

This downloads the course data into the selected cache directory. It also downloads the InstanSeg model and the Cellpose cyto3 and nuclei models.

6. Use the environment in VS Code

Open this repository folder in VS Code.

Open the Command Palette and run Python: Select Interpreter. Select the interpreter from .venv.

On macOS, Linux, or WSL, choose:

.venv/bin/python

On Windows, choose:

.venv\Scripts\python.exe

When opening a notebook, click the kernel selector in the top-right corner and choose the same .venv environment. It will be named targeted-transcriptomics-training.

Vitessce notebooks

The Vitessce hands-on notebooks use a separate environment because current harpy-vitessce releases require a newer SpatialData stack than the main course notebooks.

See vitessce/README.md for the Vitessce-specific setup.

Updating dependencies

For normal course use, do not edit uv.lock.

If dependencies in pyproject.toml are changed intentionally, update the lockfile with:

uv lock

Then recreate or sync the environment with:

uv sync --python 3.12 --locked

About

No description, website, or topics provided.

Resources

Stars

11 stars

Watchers

2 watching

Forks

Releases

Packages

Contributors

Languages