sgrna-design

On-Target and Off-Target Scoring Algorithms for CRISPR gRNAs

ALLEGRO is a synthetic biology tool that designs minimal, high activity CRISPR-Cas sgRNA libraries

Distribution-Free Uncertainty Quantification, Multi-Objective Coverage Guarantees, and Conformal Selection for Generative Genomic Design.

CRIBAR: a fast and flexible sgRNA design tool for CRISPR-based imaging

sg5 sgRNA Designer is a command-line tool for designing CRISPR sgRNAs targeting Drosophila melanogaster genes using the SpCas9/NGG system. Fetches gene sequences directly from the Ensembl REST API, scans both strands for NGG PAM sites, scores each candidate guide, and outputs a ranked list.